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Purinergic receptors stimulate Calcium transport in pancreatic duct cells
Author(s) -
Novak Ivana,
Hansen Mette Rud,
Krabbe Simon
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.796.18
Subject(s) - purinergic receptor , receptor , pancreatic duct , microbiology and biotechnology , medicine , endocrinology , pancreas , chemistry , biology , biochemistry
Normal pancreatic juice contains <1 mM concentrations of Ca 2+ , but little is known about Ca 2+ homeostasis, which may be an important function of duct epithelium. Our aim was to investigate whether duct cells from human and rat pancreas express Ca 2+ ‐transporting and Ca 2+ ‐binding proteins and whether purinergic receptors regulate Ca 2+ transport, as is known for anion transport. Expression analysis revealed that pancreatic ducts of rat and human duct cell line CFPAC‐1 (also PANC‐1 and Capan‐1) express the Na + /Ca 2+ exchanger (NCX), as well as the Ca 2+ ‐sensing receptor and the Ca 2+ ‐binding protein calbindin 9kD. These proteins localize to the luminal and lateral membranes of pancreatic duct epithelia. Ca 2+ imaging experiments showed that activation of purinergic P2X and P2Y receptors stimulated NCX in rat and human duct cells. Application of ATP to CFPAC‐1 monolayers stimulated Ca 2+ transport from the luminal to the basolateral side. Taken together, these results show that pancreatic ducts express a number of Ca 2+ ‐handling proteins and we suggest that these together with purinergic receptors may play a role in regulation of pancreatic Ca 2+ transport. Support: PhD scholarship, University of Copenhagen and DNSRC 272‐05‐0420