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Striatal interneurons in dissociated culture
Author(s) -
Schock Sarah C,
JolinDahel Kheira,
Staines William,
Arbuthnott Gordon,
GarciaMunoz Marianela
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.790.4
Subject(s) - striatum , neuroscience , cholinergic neuron , cholinergic , interneuron , gabaergic , parvalbumin , calretinin , biology , glutamatergic , medium spiny neuron , ganglionic eminence , axon , glutamate receptor , dopamine , inhibitory postsynaptic potential , receptor , biochemistry , immunohistochemistry , immunology
There are 4 interneuron types in the striatum. Three contain GABA and either parvalbumin, calretinin or NOS/NPY/somatostatin. The fourth is cholinergic. Most studies characterizing the striatum use rat organotypic cultures, in part because cholinergic interneurons don't survive in late embryonic dissociated striatal cultures. Our aim was to produce a dissociated striatal culture containing cholinergic interneurons and to characterize them after 35 days in vitro. Cholinergic neurons from E12.5 CD‐1 mice were combined with striatal neurons and cortical neurons from E14.5 CD‐1 mice using cryopreservation technical support from QBM Cell Science. We evaluated the characteristics of striatal interneurons after 35 days in vitro in cultures of striatal neurons alone or in corticostriatal cocultures. Inclusion of cortical neurons increased dendritic branching and axon terminal outgrowth of the interneurons compared to striatal cultures alone. Cortex from GFP transgenic mice enabled identification of cortical versus striatal neurons. Interneurons showed extensive GABAergic and glutamatergic innervation. Since we are able to obtain cultures with and without the cholinergic neurons we now can study the contribution of the giant aspiny neurons to the network properties of the striatum. Funded in collaboration with OISTpc.