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Mapping of serotoninergic and nonserotoninergic connections from the medullary midline to diaphragm motoneurons in the cat
Author(s) -
Rice Cory D,
Lois James H,
Yates Bill J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.783.5
Subject(s) - tph2 , brainstem , reticular formation , serotonergic , anatomy , dorsal raphe nucleus , raphe nuclei , raphe , lateral reticular nucleus , biology , reticular connective tissue , medulla oblongata , reticular activating system , neuroscience , nucleus , central nervous system , serotonin , biochemistry , receptor
Prior work in the cat showed that brainstem medial reticular formation (MRF) neurons are infected following injection of the transneuronal tracer rabies virus (RV) into the diaphragm. In the present study, we ascertained whether this population of neurons is serotoninergic. Felines were euthanized at a variety of times following injections of RV into the diaphragm, and brainstem sections were processed using antibodies to detect RV and tryptophan hydroxylase‐2 (TPH2). TPH2+ neurons were concentrated in the raphe nuclei, MRF region dorsal to the inferior olive, lateral reticular formation especially the area near the lateral reticular nucleus (LRN), and dorsal pontine reticular core. At short survival times following RV injections, only a small fraction of the TPH2+ neurons were dual‐labeled, although RV‐infected cells were present in MRF and LRN. At longer survival times, a large fraction of TPH2+ neurons in the medullary raphe nuclei, MRF and LRN were infected by RV, as were TPH2‐ cells in the same areas. However, a much smaller proportion of pontine TPH+ neurons was infected by RV. These data show that both serotoninergic and non‐serotoninergic medullary midline neurons participate in regulating diaphragm activity, mainly through multisynaptic connections.