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Effects of Metabolic Syndrome (MetS) on Male and Female Ossabaw Miniature Pig Detrusor Muscle Contractility and Compliance
Author(s) -
Francis Samuel J,
Yang David,
Sharma Richa,
Alloosh Mouhamad,
Sturek Michael S,
Packer C Subah
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.781.12
Subject(s) - contractility , medicine , compliance (psychology) , detrusor muscle , endocrinology , metabolic syndrome , smooth muscle , diabetes mellitus , psychology , social psychology
Diabetic bladder dysfunction is not understood. Previous work suggests that male MetS Ossabaw pigs have stiffer and more contractile detrusor muscle compared with lean male pigs. The purpose of the current study was to determine if altered detrusor muscle function in MetS pigs is gender dependent. Detrusor strips from male and female Ossabaw pigs maintained on an atherogenic or control diet were mounted at slack length in muscle baths. Following equilibration, muscle strips were stimulated with supramaximal electrical field stimulation (EFS). Following relaxation, cycles of incremental stretches (0.5‐1.5 mm) followed by EFS‐induced contractions were repeated every 5 minutes until maximum total tension was achieved or the tissue yielded. Normalized passive and active length‐tension (L‐T) curves were generated. Both mean passive and active L‐T curves for MetS bladder muscle are elevated above the respective control curves indicating increased contractility and decreased compliance of bladder muscle in both male and female MetS pigs. Stiffer bladders that are hypercontractile could explain both incomplete voiding and increased urinary frequency experienced in diabetes characteristic of metabolic syndrome. (D Yang is an Indy Project SEED Scholar; S Francis is an IUSM Summer Research in Academic Medicine Scholar; R Sharma was a 2007 APS Undergraduate Summer Research Fellow; Support: NIH RR013223, HL062552)