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HIF signaling during molting in Crustacea
Author(s) -
Miller Wyatt A.,
Terwilliger Nora B.,
Towle David W.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.779.4
Subject(s) - downregulation and upregulation , moulting , biology , microbiology and biotechnology , gene expression , medicine , gene , endocrinology , ecology , genetics , larva
Increasing evidence has demonstrated HIF regulation of gene activity in response to both hypoxia and non‐hypoxic stimuli. Normoxic upregulation of vertebrate HIF alpha has been reported during tissue reconstruction, vascularization, and the immune response, events also integral to the crustacean molt cycle. This study investigated the potential role of HIF alpha in mobilizing genes during molting in normoxic crabs. In premolt, the epidermis underlying the exoskeleton hypertrophies, hemocytes and reserve cells proliferate, cryptocyanin increases, and selected muscle proteins atrophy. New exoskeleton secretion occurs from premolt through postmolt. Tissue growth, vascularization and sclerotization begin in postmolt. A cohort of juvenile Dungeness crab Cancer magister was sampled daily for an entire molt cycle. Analysis of HIF alpha, arthrodial membrane protein 6, anti‐lipopolysaccharide factor, cryptocyanin 2 and B‐actin mRNA expression was carried out via real‐time quantitative PCR using SYBR GreenER qPCR SuperMix, ABI Prism 7700 instrumentation (Applied Biosystems) and non‐degenerate primers. Each gene showed a specific pattern of translational upregulation consistent with its putative role in molting. The changes in HIF alpha transcript support the hypothesis that HIF alpha is upregulated in normoxic crabs to coordinate gene expression during molting. Supported by MDIBL NIA (NBT).