Premium
Effect of pyrene exposure on skin ionic conductance in Rana pipiens and Rana catesbeiana
Author(s) -
Anderson Kristy A,
Stabenau Erich K
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.778.19
Subject(s) - rana , leopard frog , pyrene , chemistry , frog skin , amphibian , salientia , conductance , ionic bonding , grenouille , biophysics , anatomy , ion , biology , ecology , biochemistry , sodium , organic chemistry , xenopus , mathematics , combinatorics , gene
Previous studies have demonstrated that a 2‐ringed polycyclic aromatic hydrocarbon (PAH) can alter ionic movement across frog skin (Blankemeyer and Hefler, 1990). However, no information is available on the effects of complex PAHs on ionic conductance across frog skin. In the present study, Rana pipiens were exposed to saturated pyrene levels, a 4‐ringed PAH commonly found in large concentrations in aquatic environments. Following a 7‐day exposure, frogs were anesthetized, pithed, and the abdominal skin mounted into Ussing chambers with frogs Ringers solution bathing each side of the tissue. I‐V relationships were generated following clamping of the skin at ‐40, ‐20, 0, 20, and 40 Volts. The data revealed that pyrene exposure significantly reduced the ionic conductance across the leopard frog skin. Using ion replacement studies on the mucosal and serosal sides of skin from control animals, it was determined that replacing mucosal Na + and Cl − produced similar tissue conductance levels to that measured in pyrene‐exposed frogs without ion replacement. These data suggest that pyrene‐exposure significantly reduces Na + and Cl − conductance in frog tissue. Limitations of the influx of these ions into the frogs may produce osmoregulatory limitations. It is noteworthy that similar effects were not observed in R. catesbeiana . These studies were funded, in part, by a Bradley University Research Excellence grant to EKS.