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Cystathionine γ‐lyase and cystathionine β‐synthase expression and activity change with age and caloric restriction
Author(s) -
Alendy Maikel J,
Predmore Benjamin Lee,
Ahmed Khadija,
Leeuwenburgh Christiaan,
Julian David
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.776.7
Subject(s) - cystathionine beta synthase , cystathionine gamma lyase , medicine , aorta , endocrinology , enzyme , senescence , gene expression , biology , chemistry , biochemistry , gene , cysteine
Hydrogen sulfide (H 2 S), a gas signaling molecule with actions on the cardiovascular system, is endogenously produced by cystathionine γ‐lyase (CSE) and cystathionine β‐synthase (CBS). We have previously shown an increase in H 2 S signaling efficacy with age and caloric restriction (CR) in rat aorta and hypothesized that CSE and CBS expression would change similarly. Aorta and liver tissues were collected from rats of ages 8 to 38 months maintained on CR or ad libitum (AL) diets. Protein and RNA were extracted for western blotting and real time PCR, respectively. H 2 S production was determined in tissue homogenates. While age did not affect H 2 S production or RNA expression, diet did: aorta and liver H 2 S production and CSE RNA expression were higher in CR than AL animals for all ages (t‐test, p=0.041). Age and diet both affected protein expression (2‐way ANOVA, p=0.0056): in aorta, both enzymes increased with age, with expression highest in AL animals at older ages; in liver, CSE increased with age, with expression highest in CR animals at older ages, whereas CBS decreased with age with no diet affect. These data show that for aorta, enzyme expression increases with age while RNA expression does not, and CR animals with lower CSE and CBS produce more H 2 S than AL animals. However, this may not be true of non‐vascular tissues, such as liver. This suggests a decrease in enzyme function with age that may be attenuated by CR.