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Gender‐related differences in synaptic ecto‐ATPase in skeletal muscle arteries
Author(s) -
Stone Audrey Jon,
Evanson Kirk W.,
Kluess Heidi A.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.775.1
Subject(s) - medicine , anatomy , cardiology , phosphate , endocrinology , femoral artery , atpase , chemistry , biochemistry , enzyme
The purpose of this study was to determine the activity of ecto‐ATPase in the vasculature of males and females. The hypothesis was that females would have greater ecto‐ATPase activity. The left and right femoral arteries, saphenous arteries, and gastrocnemius‐ 1A arterioles (G1A) were removed from F344 rats (male n=8; female n=8). Vessel segments (2mm) were incubated at room temperature in a PiBlue Reagent for 30 minutes. Free phosphate was measured at an absorbance of 620nm. No differences were found between genders for phosphate concentration (males: 1111±517 pM; females: 1046±445 pM). There was no difference in phosphate concentration for left and right vessels (left: 1067±483 pM; right: 1091±483 pM). However, there was a significant difference in vessel type (Femoral: 1292±476 pM; Saphenous: 1160±411 pM; G1A: 784±412 pM; p<0.05). Within males, phosphate concentration differed between femoral arteries and G1A arterioles (1405± 557; 774±414 pM; p<0.05). Within females, phosphate concentration differed between femoral arteries and G1A arterioles (1179±362; 793±423 pM; p<0.05) and differed between saphenous and G1A arterioles (1165±457; 793±423 pM; p<0.05). Although gender does not appear to influence the amount of synaptic ecto‐ATPase in skeletal muscle arteries, vessel type may be influential. Research supported by Arkansas Biosciences Institute and the National Institute on Aging.