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Empirical study of transfecting early growth response gene‐1 DNA enzyme to prevent intimal hyperlasia of autogenous vein graft in rats
Author(s) -
Wang ShuQing,
Liu ChengWei,
Lv DaMing,
Kang YuMing,
Wang ShuQiu,
Wei XiaoDong,
Qin WenBo
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.774.2
Subject(s) - intimal hyperplasia , adventitia , vascular smooth muscle , vein , genetic enhancement , medicine , cell growth , gene , pathology , biology , endocrinology , biochemistry , smooth muscle
To detect the inhibitory action of early growth response gene‐1 DNA enzyme (EDRz) to vascular smooth muscle cell (VSMC) proliferation and intimal hyperplasia, with liposome as carrer, and to confirm the effect of gene therapy on stenosis and occlusion after vein transplantation. Construct early growth response gene‐1 DNA enzyme, autogenous vein graft model was established in 90 Sprage‐Dawley rats. After surgery, rats were randomly divided into at 1, 2, 6, 24 hours, 3, 7, 14, 28, 42 days groups. At 1h after graft, early growth response gene‐1 DNA enzyme was mainly located in media, adventitia and partial endothelial cells of vein graft. From 2h to 24h it was located in media of vein graft, while it was mainly located intimal of vein graft at 7d. There were not early growth response gene‐1 DNA enzyme in vein grafts at the late phase. We found that Egr‐1 protein mainly located in the medial VSMCs, monocytes and endothelium cells of vein graft at the early phase, and at the late phase no Egr‐1 protein was found in medial and neointimal VSMCs. The positive cells rate: at 2h, 6h, 24h, 3d is 15.3±4.2%, 9.7±2.4%, 6.4±1.8%, 2.3±0.2%. Conclusions: Early growth response gene‐1 DNA enzyme can reduce the expression of Egr‐1 in autogenous vein graft, it can effectively restrain VSMC proliferation and intimal hyperplasia, and then prevent vascular stenosis and occlusion after vein graft.