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Alcohol intoxication suppresses blood‐borne mediators which signal hematopoietic changes during pneumococcal pneumonia.
Author(s) -
Raasch Caroline Elizabeth,
Zhang Ping,
Neslon Steve,
Bagby Greg J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.771.5
Subject(s) - pneumonia , haematopoiesis , bone marrow , medicine , immunology , lung , stem cell , biology , genetics
Alcoholic patients with pneumonia frequently present with low leukocyte counts, predictive of poorer outcomes. Our previous studies have shown alcohol intoxication attenuates the induction of Sca‐1 surface protein in lineage − c‐kit + cells during infection, which is associated with granulocytopenia and increased mortality. To investigate how alcohol suppresses Sca‐1 induction during pneumonia, plasma collected from mice 24 h after i.p. saline or alcohol and i.t. S. pneumoniae was added to lineage − c‐kit + Sca‐1 − bone marrow cells isolated from naïve mice. After 24h in culture, cells were analyzed for Sca‐1 surface protein. Cells exposed to plasma from pneumonia infected animals demonstrated a 4.4‐fold increase in Sca‐1 + cells over control levels (p < 0.05), but no increases were seen in response to plasma from mice intoxicated at the time of infection. As Sca‐1 re‐expression contributes to the pneumonia‐induced increase in stem cell number, alcohol intoxication may suppress the hematopoietic response to pneumonia in part by impairing the release of a blood borne mediators. Supported by NIH AA07577 & AA09803.