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Alignment‐dependent regulation of vangl2, a planar cell polarity core member, in adult endothelial cells
Author(s) -
Rekapally Harish,
Stiening Chad M,
Hoying James B
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.767.2
Subject(s) - golgi apparatus , microbiology and biotechnology , cell polarity , cytoplasm , cell , downregulation and upregulation , shear stress , polarity (international relations) , biology , chemistry , materials science , genetics , gene , endoplasmic reticulum , composite material
Proper vessel inflow‐outflow polarity is important for vascular health. We hypothesized that the planar cell polarity (PCP) molecular program, which establishes polarity in embryonic tissue fields, participates in adult EC polarized behavior. We show, for the first time, that Vangl2, a core PCP member, is present in adult ECs, is expressed throughout neovascularization and is regulated by shear stress‐dependent EC re‐alignment. Under static conditions, Vangl2 is located in punctate structures within the cytoplasm of cultured human iliac endothelial cells (HIAEC). Upon exposure to 20 dynes/cm 2 for 24 hrs, Vangl2 protein expression is upregulated and appears within the Golgi complex. Continued exposure of EC to shear stress without re‐alignment maintains elevated Vangl2 expression, but Vangl2 is lost from the Golgi complex. However, when cell re‐alignment is induced during continued flow exposure, Vangl2 re‐appears in the Golgi and its expression is increased further suggesting that cell alignment and not shear stress, per se, is the stimulus for Vangl2 regulation. These findings demonstrate the active presence of the PCP program in adult endothelial cells and, given its conserved role in organizing cell signaling, may act to coordinate EC activity associated with cell polarization and, therefore, may be important in the control of vascular maturation and organization. Supported by AHA 0755710Z.