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Low density lipoprotein modulates rat mesenteric lymphatic pumping
Author(s) -
Wang Wei,
Muthuchamy Mariappan,
Zawieja David C
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.764.1
Subject(s) - lymphatic system , lymph , lymphatic vessel , endocrinology , medicine , enos , chemistry , lipoprotein , endothelium , receptor , lymphatic endothelium , biology , pathology , immunology , cholesterol , nitric oxide , nitric oxide synthase , cancer , metastasis
The unique nature & location of mesenteric lymphatics provides a special site for modulating lipoprotein transport, including low density lipoprotein (LDL). Intestinal lymph contains both enterocyte‐synthesized & plasma‐derived lipoproteins. Lipid absorption is associated with alterations in mesenteric lymph pressure & flow, implying that they coordinate lymph transport with intestinal lipid absorption by adjusting lymph pump flow. However, detailed studies of the mechanisms involved haven't been done, so we studied this process in isolated rat mesenteric lymphatics. We found: 1. Prominent expression of LDL receptors in lymphatic cells, endocytosis of LDL into lymphatic endothelium, and endothelial NO synthase (eNOS) expression in lymphatic endothelium; 2. LDL up‐regulates phasic contraction frequency and alters tone, resulting in a significant increase in the lymph pump output. Therefore we hypothesize that: LDL exposure, acting through its receptors and/or modulations of eNOS activity, provides positive feedback to the lymphatic pump via increases in the phasic contraction frequency with accompanying alterations of the tone. These studies may help correlate lymphatic function with lipoprotein metabolism and provide the basis for the prevention and treatment of the disorders related to lipoprotein metabolism. NIH HL70308 & HL80526