Premium
Sterile biofilm on intraperitoneal polymers results in local and systemic inflammation in mice
Author(s) -
Flessner Michael F,
GomezSanchez Elise,
Potter Rebecca,
Li Xiarong,
He Zhi,
Henegar Jeffrey,
Hoskins Glenn
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.762.16
Subject(s) - mesothelial cell , staining , peritoneum , biofilm , inflammation , intraperitoneal injection , pathology , connective tissue , chemistry , microbiology and biotechnology , biology , immunology , medicine , bacteria , genetics , pharmacology
To address the hypothesis that inflammatory biofilm on an intraperitoneal (ip) polymeric foreign body is associated with tissue and systemic inflammation, we inserted ip into 40 C57Bl/6 mice: 5 sterile loops of catheter made from silicone (SI), polyurethane (PU), or polyethylene (PE); controls had sham operations. After 1‐5 weeks, dis‐membranated biofilm and peritoneal tissue were cultured to demonstrate sterility and studied with electron microscopy (EM) and immunochemistry (IC). Transmission EM demonstrated macrophage, fibroblast, lymphocyte, mesothelial cells but no bacteria. Total biofilm cell density (#/cm 2 ) varied from 1‐4 x 10 4 in the first week and increased with time. Marked changes were noted in thickness, angiogenesis and peritoneal staining of TGFβ, FGF, VEGF, and αSMA =4 times that of controls. Biofilm cells were identified with IC (antibody) as macrophages (F4/80), lymphocytes (CD8), mesothelial cells (cytokeratin), myofibroblasts (aSMA, vimentin). Biofilm and extraperitoneal tissue IC staining for cytokines paralleled the alterations in the peritoneum. Control animals had minimal staining in all tissues. These data demonstrate a sterile biofilm is associated with marked structural and inflammatory changes in peritoneal and extraperitoneal tissue.