Premium
Cytotoxic effects of manganese nanoparticles using different solvent system in astrocytes and neuronal cultured cell
Author(s) -
Ali Syed F,
Boulton M C,
BraydishStolle L K,
Murdock R C,
Jiang H,
Rongzhu L,
Miltatovic D,
Aschner M,
Schlager J J,
Hussain S M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.759.3
Subject(s) - neurotoxicity , chemistry , cytotoxicity , oxidative stress , cytotoxic t cell , biophysics , manganese , nanoparticle , cell culture , astrocyte , nuclear chemistry , mtt assay , cell , microbiology and biotechnology , biochemistry , toxicity , nanotechnology , materials science , biology , in vitro , endocrinology , organic chemistry , genetics , central nervous system
Recently we have shown that nanoparticles such as Mn, Ag and Cu can be neurotoxic by generating ROS and depleting dopamine concentration in PC‐12 cells. We synthesized Mn nanoparticles (Mn‐NP) using different solvents. These NP were subsequently characterized using DLS, Laser Doppler Velocimetry and TEM. Mn‐NP when reduced with different solvents produced semi‐spherical, poly‐dispersed particles with an average size of 97.6 nm in tea, 32 nm in isopropyl alcohol, and 23 nm in caffeine. In the present study, we evaluated the cytotoxicity of Mn‐NP using astrocytes and neuronal cultured cell. Mn‐NP (5‐40 ug/ml) produced a concentration‐dependent decrease in MTT, and increased LDH release from cultured astrocytes and neuronal cultured cells. Mn‐NP also produced a time‐dependent increase in the expression of nuclear factor erythroid 2‐related factor 2 (Nrf2). Morphological evaluation showed a time‐dependent effect of Mn‐NP on cell morphology. The most interesting effect was found in cells treated with Mn‐NP in tea. Eight hrs post‐treatment, astrocytes appeared deformed and increased in size by 24 hrs. However, with other media, the effects were not as pronounced. Similar effects were found in neuronal cultures, where Mn‐NP in tea produced the most pronounced dysmorphic effects. These data suggest Mn‐NP effectively produce neurotoxicity by affecting astrocytic and neuronal functions, most likely by generating oxidative stress and neurotoxicity.