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Quantification of Blood Alcohol Concentration Using Alcohol Oxidase Dipsticks
Author(s) -
Petroni Daniel H,
George William J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.757.5
Subject(s) - chromatography , alcohol , absorbance , ethanol , chemistry , alcohol oxidase , standard curve , biochemistry , pichia pastoris , gene , recombinant dna
Alcohol detection dipsticks have been utilized as a qualitative test to detect the presence of alcohol in oral fluids. The present study was conducted in order to see whether alcohol oxidase dipsticks (Chematics) could be utilized as a quantitative tool to measure blood alcohol concentrations (BAC). Human saliva was utilized and spiked with ethanol standards to create a final solution with concentrations including 200, 100, 50 and 25 mg/dl of ethanol. Verification of these concentrations was done on a Shimadzu 14‐A Gas Chromatograph, equipped with a Supelco 5% Carbowax packed column. The dipsticks were exposed to the ethanol‐saliva solution and placed within a Biorad Gel Doc XR analyzer under fluorescent white light. The absorbance density of the reaction band was then measured using Biorad's Quantity One analysis software (Ver. 4.6.2) and a standard curve was plotted. A linear regression analysis was conducted between ethanol concentration and absorbance density. A significant correlation was found with an r squared value of 0.9917. These results indicate the potential use of alcohol oxidase dipsticks as a non‐invasive quantitative tool to measure blood alcohol levels.