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Estimate of fibrosis in carbon tetrachloride induced chronic liver injury in rat by breath test with L‐[1‐13C] phenylalanine
Author(s) -
Yan Weili,
Qu Xinhua,
Huang Xiaolu,
Huang Gang,
Xiong Ping
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.741.10
Subject(s) - carbon tetrachloride , excretion , breath test , chemistry , medicine , liver function , liver injury , gastroenterology , liver biopsy , liver function tests , endocrinology , pathology , biopsy , organic chemistry , helicobacter pylori
Liver malfunction is a major problem in the world. Traditional diagnoses which are based on indices of blood biochemistry and liver biopsy have limitations and disadvantages. Developing new non‐invasive methods to accurately assess liver function is always an interesting topic. The objective of this study is to investigate the relationship between the parameters of breath test and the histological grading, and provide valid parameters for the evaluation of liver function. We injected L‐[1‐13C]phenylalaline (13C‐Phe) into rats in the control group (n=8) and carbon tetrachloride (CCl4 ) induced chronic injury group (n=21) through tail vein, quantified the 13CO2 using air isotope ratio mass spectrometry, and examined histological changes of rat livers. We tested 12 parameters and found that only unit‐liver‐weight (LW) parameters, including maximum unit abundance (13Cmax/LW), unit abundance at 2 min and 7 min (13C2/LW and 13C7/LW), cumulative unit 13C excretion in 10 min and 30 min (AUC10/LW and AUC30/LW) and unit 13C excretion rate constant (PheBT‐k/LW) were significantly affected in the chronic injury group. Our findings indicate that the breath test using 13C‐Phe (PheBT) together with the unit‐liver‐weight (LW) parameters can quantitatively reflect the degree of fibrosis in the livers of chronically injured rats.