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Application of FISH Technique in Invasive Breast Cancer Diagnosis
Author(s) -
Salameh Yasmeen M.,
AlKahlout Basema I,
Bargal Hala W.,
Afifi Nahla M.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.740.2
Subject(s) - breast cancer , immunohistochemistry , medicine , fish <actinopterygii> , cancer , oncology , estrogen receptor , invasive ductal carcinoma , gynecology , pathology , biology , fishery
Breast cancer is the leading cause of cancer death among women worldwide. The aim of this study is to improve the prognosis of Breast Cancer in women living in State of Qatar by accurate diagnosis. In the study 70 Breast Cancer patients (year 2000 ‐ 2006) were included. It was found that the mean diagnostic age of breast cancer patients were (46.9 ± 10.4), the majority (48.4 %) of patients was obese (P‐value 0.006) and all of them were positive for estrogens (E) and progesterone (P) receptors. Invasive Ductal Carcinoma (IDC) was the most common type found (93.3 %). Immunohistochemical investigations revealed 30% of samples were negative for the expression of (E) & (P) Receptors. On the other hand HER‐2/neu testing by Immunohistochemistry showed that 22.9 % of the subjects were diagnosed as negative for the HER‐2/neu protein and 76.1 % subjects with positive HER2/neu receptor (54.9 % +2 expressing, 22.2 % +3 or higher) By FISH application to the selected cases, of the total Border line area of IHC tested for HER2 (+2 expression), we have found 60% of them were actually positive for the gene amplification, while 24% were found to be negative. Unfortunately 16% of the +2 expressing cases couldn't be determined for HER2 amplification due excessive denaturation. As FISH is more accurate than IHC in diagnosis of HER‐2 positive cases it's recommended to use FISH in diagnosis to make better prognosis and further training to master FISH technique to avoid any technical error. This study was funded by QU & UREP.