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Accumulation of chromium and histological findings on Hybro chickens liver treated with Cr(VI) and vitamin C
Author(s) -
Gashi Agim,
Halili Fetah,
Bajraktari Ismet,
Sopa Ramadan,
Bislimi Kemajl,
Fetahu Shukri,
Behluli Avni,
GrapciKotori Linda
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.739.13
Subject(s) - vacuolization , chromium , chemistry , h&e stain , medicine , vitamin c , bioaccumulation , lipofuscin , vitamin , staining , pathology , biochemistry , environmental chemistry , organic chemistry
In this study the role of vitamine C in the acumulation of chromium in the liver and liver lesions of Hybro chicken treated with Cr (VI) were investigated. The chickens were divided into 5 groups (I‐V), with 10 individuals which and were treated orally with different concentrations of chrome (VI) and 10 mg/kg b.w vitamin C for 180 days. The preparation of tissues for the pathological microscopy was done according to the standard procedure whereas the staining was done with hemathoxylin‐eosin. The Cr concentration on the liver was determined by atomic absorbtion spectroscopy (AAS) via UNICAM 929‐AA type spectrometer. The outcome of the research indicates that the six‐month ingestion of two different doses of Cr (VI) results in light histopathological changes in the periportal and pericentrilobular zone of the liver. The fatty vacuolization of hepathocites, dilatation of centrilobular vein, the dilated sinusoids with erythrocytes, proliferation of biliary tubules, the lymphoplasmocitary infiltrates etc. are visible. The protective role of Vitamin C on the applied dose was not noticed. Vitamin C combined with Cr (VI) doses amplifies the bioaccumulation of Cr on the liver to a considerable degree, compared not only with control group of chicken but as well as with the chicken treated only with Cr (VI) . The investigation results show that the bioaccumulation of chrome and the histological fidings of the liver depend on the dose, time of action and the combination of Cr with Vitamin C.