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Kinetics of myogenic progenitor cell (MPC) expansion and bone marrow‐derived cell recruitment in skeletal muscle regeneration
Author(s) -
Martinez Carlo Obet,
McManus Linda M,
Shireman Paula K
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.739.1
Subject(s) - progenitor cell , regeneration (biology) , bone marrow , microbiology and biotechnology , cd34 , flow cytometry , skeletal muscle , cardiotoxin , stem cell , progenitor , biology , cell , immunology , chemistry , anatomy , biochemistry
Muscle regeneration requires myogenic progenitor cells (MPC) and recruitment of bone marrow (BM)‐derived cells. MPC (CD34 + /CD45 − /Sca‐1 − ) and BM‐derived progenitor cells (CD45 + /Sca‐1 + ) isolated from injured muscle undergo myogenic differentiation in vitro . However, the kinetics of MPC expansion and BM‐derived cell recruitment to injured muscle are poorly understood and were quantitated in tibialis anterior muscle using flow cytometry after cardiotoxin (CTX) injury in wild type (WT, C57Bl/6J) male mice. Maximal MPC expansion occurred 3 days post‐CTX and BM‐derived progenitor and immune cell recruitment exhibited similar kinetics; few cells present at baseline with maximal accumulation 3 days post‐CTX. MPC expansion and BM‐derived cell recruitment occurred within days of injury and demonstrated similar accumulation kinetics suggesting interactions between MPC and BM‐derived cells may be crucial to skeletal muscle regeneration. A better understanding of interactions between different cell types could result in improved strategies for muscle regeneration and tissue engineering. Supported by Veterans Administration and HL074236 and HL090196.