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Regulation of RIP140 expression by microRNA
Author(s) -
Lin YaLun,
Tsai NienPei,
Wei LiNa
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.730.1
Subject(s) - microrna , three prime untranslated region , untranslated region , regulator , microbiology and biotechnology , endogeny , translational regulation , messenger rna , transcriptional regulation , translation (biology) , regulation of gene expression , post transcriptional regulation , transcription factor , chemistry , gene , biology , biochemistry
Receptor interacting protein 140 (RIP140) is a versatile transcription co‐regulator that plays critical roles in gene regulation. Its expression and regulation are less understood. In this study we examined the expression of RIP140 and identified its 5¡¦ un‐translated region (5¡¦ UTR). We found that its 5¡¦‐UTR could enhance RIP140 translation and be targeted by microRNA‐346 (miR‐346). By manipulating the endogenous miR‐346 level in P19 embryonic carcinoma cells, a functional role for miR‐346 in up‐regulation of RIP140 protein was revealed. This is the first study demonstrating the expression and regulation of endogenous RIP140 protein in animal tissues and cell lines, and revealing the unique regulation of RIP140 expression at the post‐transcriptional level by miR‐346 that targets the 5¡¦ UTR of RIP140 transcript.