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Effect of Omega‐3 fatty acid supplementation and ALOX5 promoter variants on Lipid Profiles in African‐Americans
Author(s) -
Armstrong Patrice,
Allayee Hooman,
Kelley Darshan,
Hartiala Jaana,
Stephensen Charles B.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.724.3
Subject(s) - very low density lipoprotein , fish oil , placebo , medicine , genotype , apolipoprotein b , endocrinology , lipoprotein , biology , cholesterol , chemistry , gene , biochemistry , fish <actinopterygii> , alternative medicine , pathology , fishery
The objective was to determine the effects of omega‐3 fatty acid supplementation and arachidonate 5‐lipoxygenase (ALOX5) gene variants on plasma lipids and lipoprotein particle profiles (i.e. LDL, HDL, & VLDL). We hypothesized that promoter variants in the ALOX5 gene may influence baseline levels and response to supplementation. The study enrolled 116 healthy African‐Americans subjects with specific deletion variants in the ALOX5 promoter (70% female, 20 ‐ 59 yr). This community‐based, double‐blind, controlled intervention trial randomized subjects within genotype in a 6 x 2 design (6 ALOX5 genotypes; allocated to either EPA/DHA supplement or placebo); 98 completed the study. The intervention group received 5.0 g/d fish oil concentrate which contained 3.0g/d EPA/DHA and the placebo group received 5.0 g/d corn/soybean oil for 6 weeks. Supplementation affected total cholesterol and total triglycerides, as well as HDL and VLDL subfractions. In addition, significant differences were seen in VLDL subfractions among ALOX5 genotypes. Our results confirm that EPA/DHA significantly affect plasma lipid profiles and suggest that ALOX5 genotype modifies this response in healthy African‐American adults. This research was supported by the Pfeiffer Foundation, NIH grants R21AT003411, RO1HL079343, & P60MD0222. Grant Funding Source National Institutes of Health