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Unsaturated fatty acids diminish elevated adipocyte fatty acid binding protein (aP2) expression by lipopolysaccharide in macrophages
Author(s) -
Park YoungKi,
Lee JiYoung
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.724.18
Subject(s) - lipopolysaccharide , adipocyte protein 2 , fatty acid , chemistry , fatty acid binding protein , biochemistry , western blot , adipocyte , tumor necrosis factor alpha , northern blot , unsaturated fatty acid , incubation , gene expression , biology , endocrinology , adipose tissue , gene
Expression of aP2 in macrophages and its role in pro‐inflammation have been reported. To evaluate the effect of fatty acids on the expression of aP2 in macrophages, murine RAW 264.7 macrophages were treated with individual fatty acid, including 14:0, 16:0, 16:1, 18:1, 18:2, 18:3, 20:4, 20:5 and 22:6 (100 μM), complexed with BSA (2.5:1 of fatty acid to BSA molar ratio) for 12 hours. After the fatty acid incubation, lipopolysaccharide (LPS, 100 ng/ml) was added to the media for additional 18 hours. Quantitative realtime PCR analysis was performed to measure aP2 mRNA abundance. LPS significantly elevated aP2 mRNA abundance by ~8‐fold. The increased aP2 expression by LPS was abolished by all the unsaturated fatty acids tested whereas saturated fatty acids did not elicit the same response. Western blot analysis also confirmed that the elevated aP2 protein level by LPS was diminished by unsaturated fatty acids. Concomitantly, unsaturated fatty acids reduced the expression of pro‐inflammatory genes, including tumor necrosis factor α, cyclooxygenase‐2, interleukin‐1β and interleukin‐6, in LPS‐activated macrophages. The results suggest that anti‐inflammatory property of unsaturated fatty acids could be mediated, at least in part, by down‐regulating aP2 expression in macrophages.