Premium
Proteomic analysis of pancreas in a polygenic mouse model of type 2 diabetes
Author(s) -
Wyatt Brantley,
Hall Lorin,
Kim Jung Han
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.724.11
Subject(s) - isoelectric focusing , pancreas , proteome , isoelectric point , proteomics , gene , heat shock protein , biology , trypsin , type 2 diabetes , diabetes mellitus , microbiology and biotechnology , chemistry , biochemistry , endocrinology , enzyme
TALLYHO/JngJ (TH) mice are a polygenic model for type 2 diabetes. We conducted a proteomic analysis in pancreas using 2D‐DIGE and mass spectrometry to identify molecules pathologically relevant to the diabetes in TH mice. Total protein was extracted from pancreas of TH and C57BL/6J mice and labeled with CyDye. Isoelectric focusing in the first dimension was conducted at pH 3‐10 to separate proteins based on pI, and in the second dimension in 8‐14% gradient SDS‐PAGE to separate proteins based on size. Differentially expressed protein spots were excised, digested with trypsin and subjected to MALDI‐TOF mass spectrometry. We observed changes in protein amounts or evidence of modifications in antioxidant defense proteins and heat shock proteins in TH mice. Proteins involved in growth and survival of pancreatic beta‐cells were also identified. In summary, the proteins identified in this study may provide links of molecular processes to the development of diabetes in TH mice, and their respective genes may serve as candidates for susceptibility genes in this polygenic model. Supported in part by NIH/NIDDK‐1R01DK077202‐01A2, AHA‐0855300E, & pilot and feasibility grant from the UT Obesity Research Center.