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Marginal Vitamin B6 Deficiency Affects Fatty Acid Profiles in Healthy Men and Women
Author(s) -
Zhao Mei,
Lamers Yvonne,
Ralat Maria A,
Gilbert Lesa R,
Keeling Christine E,
Stacpoole Peter W,
Gregory Jesse F
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.722.11
Subject(s) - medicine , chemistry , docosahexaenoic acid , endocrinology , vitamin d deficiency , eicosapentaenoic acid , phospholipid , fatty acid , arachidonic acid , vitamin d and neurology , polyunsaturated fatty acid , biochemistry , biology , membrane , enzyme
Vitamin B 6 (VB 6 ) deficiency alters fatty acid (FA) profiles of rat tissue lipids. The effects of VB 6 deficiency on FA profiles in humans are not well understood. The aim of our study was to determine effects of VB 6 deficiency on FA profiles of various blood fractions including plasma, red cells and lymphocytes. Healthy subjects (n=13) received a 2‐d adequate diet followed by a 28‐d VB 6 restricted diet (0.5 mg /d) to induce a marginal VB 6 deficiency (plasma pyridoxal‐5′‐phosphate 20‐30 nM). Plasma total FA, red cell and lymphocyte membrane FA were analyzed by gas chromatography/flame ionization. After 28d of VB 6 restriction, plasma arachidonate (C20:4 n‐6) and docosahexaenoic acid (C22:6 n‐3) concentration decreased significantly from (mean ± SD) 533 ± 72 to 479 ± 59 µM (p=0.006) and from 111 ± 25 to 98 ± 20 µM (p=0.044). The ratio of arachidonate/linoleate, (C20:4 n‐6)/(C18:2 n‐6), in plasma also significantly decreased from 0.23 ± 0.04 to 0.20 ± 0.04 (p<0.001). For red cell membrane lipids, the percentage of oleate (C18:1 n‐9) and eicosapentaenoic acid (C20:5 n‐3) significantly decreased from 18.9 ± 1.3 % to 18.6 ± 1.1% (p=0.023) and from 0.45 ± 0.12% to 0.41 ± 0.12% (p=0.042). There were no significant changes in FA profiles of membrane lipids in lymphocytes. In conclusion, marginal VB 6 deficiency has subtle but significant effects on FA profiles in humans. Supported by NIH grant DK072398 and GCRC grant M01‐RR00082.