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Green tea (GT) extract enhances the biliary secretion of benzo[a]pyrene (BaP) in rats
Author(s) -
Kim Juyeon,
Noh Sang K.,
Seo Yunjung,
Koo Sung I.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.721.7
Subject(s) - green tea extract , triolein , chemistry , secretion , medicine , benzo(a)pyrene , triglyceride , endocrinology , cholesterol , food science , biology , green tea , biochemistry , carcinogen , lipase , enzyme
Recently, we have shown that GT extract (GTE) lowers significantly the lymphatic absorption of BaP in rats. The present study was conducted to examine the effect of GTE on the biliary secretion of BaP in rats. Female rats were fed an AIN‐93G diet with or without (control) GTE at 5 g/kg diet for 4 wk. At 4 wk, rats with bile cannulae were infused at 3.0 mL/h for 8 h via a duodenal catheter with a lipid emulsion containing 1.0 mg BaP labeled with 14 C ( 14 C‐BaP), 452 μmol triolein, 20.7 μmol cholesterol, and 3.1 μmol α‐tocopherol (αTP), and 396.0 μmol Na‐taurocholate in PBS buffer (pH, 6.4). Bile was collected via a bile cannula hourly for 8 h. No significant difference was observed in bile flow for 8 h between the groups. However, the biliary secretion of 14 C‐BaP was significantly increased in rats fed GTE, compared with controls. The rates of 14 C‐BaP secretion for 8 h were 24.24 ± 0.43 %dose in rats without GTE and 31.51 ± 0.83 %dose in rats with GTE. The results indicate that GTE has a stimulatory effect on the biliary secretion of BaP in rats. The mechanism(s) by which GTE increases BaP secretion remains to be determined. Supported by the Korea Research Foundation Grant funded by Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF‐2006‐003‐C00312)