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Comparison of conjugated linoleic acid (CLA) and conjugated nonadecadienoic acid (CNA) with regard to lipid metabolism in mice
Author(s) -
Park Yooheon,
Rhee Siyeon,
Park Yeonhwa
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.717.30
Subject(s) - conjugated linoleic acid , fatty acid synthase , glut4 , endocrinology , medicine , acetyl coa carboxylase , lipid metabolism , white adipose tissue , adipose tissue , chemistry , peroxisome , peroxisome proliferator activated receptor , fatty acid , lipoprotein lipase , linoleic acid , glucose transporter , biology , receptor , biochemistry , insulin , enzyme , pyruvate carboxylase
It has been reported that conjugated nonadecadienoic acid (CNA) exhibits modulation of body fat with mechanisms that are similar to those of CLA. In this current investigation, we compared the effects of CNA and CLA on various feeding period, in particular studying lipid metabolism from white adipose tissue. One hundred thirty 10 week‐old male CD‐1 mice were fed a semi‐purified powdered diet with 20% total fat based on the AIN‐93 diet. After a 2‐week adaptation period, mice were randomly assigned into control and 2 treatment groups (0.5 w/w% CLA and 0.1 w/w% CNA). Relative mRNA expression of peroxisome proliferator‐activated receptor‐γ (PPAR‐γ), acetyl‐CoA carboxylase (ACC), fatty acid synthase (FAS), hormone‐sensitive lipase (HSL), and glucose transporter 4 (GLUT4) were determined at week 0, 2, 4, 8, and 12 by real time PCR from the white adipose tissue. The effect of CNA treatment on total adipose tissue mass was less effective than CLA over time. HSL mRNA expression was significantly increased by CNA at week 8 and 12, while CLA had no effect at any time point. PPAR‐γ expression was significantly decreased by both CNA and CLA after week 2 and 4, respectively, and the decreased expression of PPAR‐γ was maintained for 12 weeks. FAS expression was enhanced by CNA during week 4 and 8 and by CLA at all time points. ACC expression was increased by CNA longer than 8 weeks, while CLA increased it at all time points. GLUT4 expression was decreased at week 8 by CNA but not by CLA. In conclusion, CNA reduces body fat mass similar to CLA. Although CNA shares mechanisms with CLA on body fat control and lipid metabolism, there were differences in the effects on lipid metabolism between CNA and CLA over time. Supported by AHA SDG and ILSI North America