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Role of grape powder extract (GPE), quercetin (QUE), and resveratrol (RSV) in attenuating markers of inflammation in primary cultures of human (pre)adipocytes
Author(s) -
Chuang ChiaChi,
Bumrungpert A.,
Kennedy A.,
Martinez K.,
WalkerWest T.,
McIntosh M.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.717.16
Subject(s) - tumor necrosis factor alpha , inflammation , resveratrol , mapk/erk pathway , adipose tissue , activator (genetics) , insulin resistance , chemistry , white adipose tissue , monocyte , endocrinology , biology , kinase , medicine , immunology , insulin , biochemistry , gene
Obesity‐associated inflammation in white adipose tissue (WAT) is strongly implicated in the development of insulin resistance. GPE is rich in phenolic phytochemicals like QUE and RSV that possess anti‐inflammatory properties. Thus, we examined the ability of GPE, QUE, and RSV to attenuate tumor necrosis factor (TNF)‐α ‐mediated activation of mitogen‐activated protein kinases (MAPK), nuclear factor kappa B (NFκB) and activator protein (AP)‐1 and induction of inflammatory genes in primary cultures of human (pre)adipocytes. We found that GPE, QUE, and RSV attenuated TNF‐α ‐induced expression of inflammatory adipocytokines [i.e., interleukin (IL)‐6, IL‐1β, IL‐8], markers of macrophage recruitment into WAT [i.e., monocyte chemoattractant protein (MCP)‐1], and negative regulators of insulin sensitivity [i.e., protein tyrosine phosphatase (PTP)‐1B]. Furthermore, we found that GPE and QUE attenuated TNF‐α ‐activated MAPK (i.e., ERK1/2, JNK1/2), NFκB, and AP‐1 (i.e., c‐Jun). RSV, however, only modestly attenuated TNFα‐activation of NFκB and c‐Jun, and had no effect on TNF‐α ‐activation of ERK1/2 and JNK1/2. Taken together, these data suggest that GPE, QUE, and RSV attenuate TNF‐α ‐mediated inflammation in primary cultures of human (pre)adipocytes by different upstream mechanisms. (Funded by the WB Kellogg Institute for Food and Nutrition Research; Grape Powder supplied by the CA Table Grape Com.) Grant Funding Source WB Kellogg Institute for Food and Nutrition Research