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Plum polyphenolics decrease oxidative stress and inflammation in vascular endothelial cells
Author(s) -
Townsley Emily D.,
Noratto Giuliana,
Angel Gabriela,
Del Follo Armando,
Byrne David,
MertensTalcott Susanne
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.717.12
Subject(s) - umbilical vein , oxidative stress , antioxidant , inflammation , polyphenol , chemistry , reactive oxygen species , gallic acid , human umbilical vein endothelial cell , biochemistry , pharmacology , biology , immunology , in vitro
Previous studies have demonstrated that plums are rich in antioxidant polyphenolics which may have anti‐inflammatory effects. In this study we determined the anti‐oxidant and anti‐inflammatory effects of polyphenolics extracted from ″Black Splendor″ (BS), a commercial variety of plum ( Prunus salicina ). BS concentrations of up to 10 mg gallic acid equiv./L did not significantly alter cell proliferation and therefore were selected for the determination of antioxidant and anti‐inflammatory effects in human umbilical vein endothelial cells (HUVEC). Reactive oxygen species (ROS) were induced with 10mM glucose for 30 min at 37°C and BS (≥2 mg/L) decreased the induced oxidative stress by up to 70% upon treatment . Treatment of HUVEC cells with 10mM glucose significantly increased messenger‐RNA expression of inflammation biomarkers IL‐6, IL‐8, and NF‐κB. An increase in transcription factor NF‐κB up‐regulates other pro‐inflammatory cytokines leading to increased vascular inflammation. Upon treatment with BS (4 mg/L), IL‐6, IL‐8, and NF‐κB were decreased by 4.4, 7.4, and 3.1 fold, respectively. This data suggests that antioxidant polyphenolics found in plum extracts help control inflammation and inhibit oxidative stress in human umbilical vein endothelial cells. Grant Funding Source Texas A&M University Vegetable and Fruit Improvment Center