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Plasma and interstitial fluid (ISF) bioavailability of green tea catechins
Author(s) -
Gerber Sophie,
Myracle Angela,
Peters Catrina,
Ferruzzi Mario,
Janle Elsa
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.716.4
Subject(s) - bioavailability , pharmacokinetics , chemistry , pharmacology , ascorbic acid , catechin , polyphenol , antioxidant , food science , medicine , biochemistry
Green tea (GT) catechins are antioxidants which have many potential health benefits. Their bioavailability has previously been evaluated only by plasma pharmacokinetics, but target tissue concentrations are likely more important to delivery of specific health benefits. The aim of this study was to demonstrate the relationship between plasma and ISF pharmacokinetics of catechins from GT. Sprague Dawley rats were implanted with ultrafiltrate probes, for sampling of ISF, and femoral catheters. Rats were dosed by oral gavage with 50 mg of GT extract and 10 mg ascorbic acid. The ascorbic acid was added to increase bioavailability. Blood samples were collected at baseline and 0.5, 1, 2, 4, 6, and 8 hours. ISF was collected two hours before gavage and every two hours post gavage for 16 hours. Catechins in the plasma and ISF were extracted with 0.01 % butylated hydroxytoluene in ethyl acetate and analyzed by HPLC. The results showed that epigallocatechin peaked in the plasma between one and two hours and epicatechin peaked in the plasma at half‐an‐hour. Both catechins peaked in ISF about two hours post gavage. This data suggests that plasma and ISF pharmacokinetics may not be the same for all catechins. Some catechins may provide benefits to target tissues for a longer duration of time than indicated by plasma pharmacokinetics. Supported by NIH, Office of Dietary Supplements and NCCAM Grant P50 AT 00477.