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Phenolics from mango (Mangifera indica L.) suppress growth in different cancer cells, targeting pro‐apoptotic and cell cycle control proteins
Author(s) -
Bertoldi Michele Correa,
Noratto Giuliana D.,
Talcott Steve Terel,
MertensTalcott Susanne U.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.716.11
Subject(s) - lncap , cell cycle , apoptosis , mangifera , chemistry , gallic acid , flow cytometry , cell cycle checkpoint , cell growth , cell , growth inhibition , ic50 , cancer cell , microbiology and biotechnology , antioxidant , biochemistry , cancer , biology , botany , in vitro , genetics
Gallotannins are phenolics in mango which have shown anti‐cancer activity. The objective was to evaluate the anti‐prolioferative activity of mango phenolics in cancer cells. The cultivars Haden (Hd) and Ataulfo (At) were selected based on phenolic content and antioxidant activity. The anti‐proliferative and pro‐apoptotic activities of phenolics extracts were tested in human Molt‐4 leukemia, A‐549 lung, MDA‐MB‐231 breast, SW‐480 colon and LnCap prostate cancer cells. Additionally, gene expression was investigated by RT‐PCR and cell cycle kinetics by flow cytometry. Hd inhibited the growth of SW‐480, Molt‐4, LnCap, MDA‐MB‐231, and A‐549 cells (IC50 = 2, 2.5, 7, 8.3, and 10.5 mg gallic acid equiv./L, respectively) as determined by cell counting at 72h, with SW‐480 cells being most sensitive to the treatments. In general, At extracts were less effective than Hd. HPLC‐PDA analysis of extracts revealed that Hd and At mainly contained gallotannins, though Hd contained higher concentration of lower molecular weight phenolics. The growth inhibition of Hd polyphenolics (15mg/L) in MDA‐MB‐231 was associated with increased expression of pro‐apoptotic caspase‐8 and Bax by 1.2 and 1.5 fold, respectively. Furthermore, Hd (up to 15mg/L) induced cell cycle arrest in the S and G 2 /M phase. Overall, mango phenolics may possess chemopreventive activities by targeting pro‐apoptotic genes and inducing cell cycle arrest. Grant Funding Source National Mango Board