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Molecular Characterization of Antibiotic‐Producing Bacteria from Brooklyn Soils.
Author(s) -
Fadael Rudgie,
Bolnet Carolle
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.713.2
Subject(s) - 16s ribosomal rna , genbank , biology , bacteria , microbiology and biotechnology , streptomyces , genetics , gene
In our previous studies, physiological and morphological methods were used in an attempt to screen out new potent antibiotic producers from different locations in Brooklyn, NY: Manhattan Beach (MB), Coney Island Beach (CIB), Prospect Park (PP) and Downstate (DS). Cultures from CIB soil not only displayed the most bacterial diversity but also revealed colonies showing signs of antibiosis. Among these cultures, we were able to identify bacteria of the genus Streptomyces whereas the others could not be identified accurately by those two methods. In this study, we used molecular tools to characterize unidentified bacteria from CIB. DNA was extracted from pure cultures and amplified using a nested PCR designed for the 16S rRNA gene that significantly reduces the amount of non specific amplification. The cleaned PCR products were sequenced by using Big‐Dye chemistry and the ABI3730 automated sequencer. Using BLAST, the obtained sequences were compared to bacterial 16S rDNA sequences in GenBank. Analyses of the results revealed a 99% nucleotide similarity to the 16S rDNA sequence of Streptomyces hygroscopicus very well known as a producer of antimicrobial agents such as rapamycin, ascomycin as well as the immunosuppressant FK506.