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Analgesic properties of an indigenous polyherbal preparation
Author(s) -
IROANYA ONYEKACHI OGBONNAYA,
OKPUZOR JOY ELISABETH,
MBAGWU HERBERT,
OJOBO PETER DOUGLAS
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.712.2
Subject(s) - analgesic , hot plate , pharmacology , acetic acid , chemistry , vernonia amygdalina , in vivo , ocimum gratissimum , traditional medicine , morphine , aqueous extract , median lethal dose , medicine , toxicity , biochemistry , biology , microbiology and biotechnology , mechanical engineering , organic chemistry , engineering
The Pharmacological effect of the polyherbal preparation of an aqueous extract obtained from the leaves of Gongronema latifolia, Vernonia amygdalina and Ocimum gratissimum was evaluated for its analgesic effect and acute toxicity. The effects of the preparation was studied using various in vivo models of analgesic effects (central and peripheral acting analgesics) in mice and the potent inhibitory activity against acetic acid induced mouse writhing , hot plate and formalin induced pain tests. In control mice, the reaction time to the mouse writhing assay was 91 ± 12.00. The mice injected with 10mg\kg of 0.6% acetic acid (i.p.) presented 68.9% writhes in 15 minutes while treatment with the extract at 2000mg\kg, 4000mg\kg and 8000mg\kg of the extract significantly (P <0.05) and dose dependently reduced the number of writhes by 61.9% , 65.7% and 66.6% writhes respectively in 15 minutes. The extract demonstrated a dose dependent relationship in the hot plate and formalin induced pain assay. The highest dose of the extract presented a significant inhibition 138.5% in comparism to that produced by Morphine 180.8% for the hot plate assay while for the formalin induced pain assay, the highest dose exhibited a significant inhibition of 46% while Acetylsalicylic acid produced a significant inhibition of 49%. The single oral dose of the Polyherbal extract at 16000mg\kg (p.o.) and 2500mg\kg (i.p.) did not produce mortality or significant change in the general behaviour of the mice. The data obtained shows that this polyherbal preparation possesses analgesic activity.