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The effects of selected plant methanol extracts on rat's brain lipid peroxidation and mouse 3T3‐L1 preadipocytes cell proliferation in vitro
Author(s) -
Abanto Oliver Diaz,
Woo HyunJoo,
Hong Heeok,
Hwang SeongGu
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.711.4
Subject(s) - lipid peroxidation , tbars , antioxidant , cell growth , chemistry , pharmacology , biology , biochemistry
Previous studies indicated that some functional foods which have medicinal and antioxidant activities also stimulate growth of preadipocytes, posing potential health risk to obesity‐prone individuals. The present study was conducted to screen and determine the effects of selected plant methanol extracts on in vitro lipid peroxidation and growth stimulation of preadipocyte cells. The antioxidant effect of each extract (D. asper, C. officinale, U. pumila, R. vulubilis, and A. sinensis) against lipid peroxidation was measured using TBARS analysis. Mouse 3T3‐L1 preadipocyte cells were treated with 0, 50, 100, 200, 400, and 800 ppm of each plant extract, and cell proliferation was measured after 24 and 48h incubation periods using tetrazolium salt (WST) assay. All of the plant extracts tested demonstrated antioxidant activities against lipid peroxidation. The U. pumila showed the strongest antioxidant activity among other extracts. The U. pumila caused arrest of 3T3‐L1 cell proliferation dose dependently, but D. asper, C. officinale and A. sinensis stimulated cell proliferation. The R. vulubilis did not stimulate nor caused growth arrest of 3T3‐L1 cells. Our findings suggest that U. pumila can potentially be used as functional antioxidant and at the same time as obesity control agent.