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Cloning and expression of parasitic mitogen‐activated protein kinase
Author(s) -
Apablaza Pablo,
Shah Swati,
Patel Samta,
Sierkierka John,
Dyer James
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.710.6
Subject(s) - kinase , protein kinase a , biology , p38 mitogen activated protein kinases , microbiology and biotechnology , heterologous expression , cloning (programming) , mitogen activated protein kinase , heterologous , clone (java method) , toxoplasma gondii , parasite hosting , plasmodium falciparum , biochemistry , immunology , gene , antibody , malaria , recombinant dna , world wide web , computer science , programming language
Mitogen‐activated protein kinases (MAPKs) are involved in a wide variety of processes in many types of cells and in many different organisms. Human MAPKs are known to be responsive to stress stimuli and are involved in apopotosis and cell differentiation. Parasite and plant MAPKs are also involved in stress responses. These MAPKs have been identified in the Apicomplexan parasite Toxoplasma gondii and also in the malarial parasite Plasmodium falciparum. The purpose of this research is to clone and express these MAPKs in a heterologous expression system so that futher biochemical characterization of the protein may be done.