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Role of MAPK/AP‐1 Signaling in the protection of 2‐choloroethyl ethyl sulfide (CEES) ‐ induced lung injury by antioxidant liposome
Author(s) -
Mukhopadhyay Sutapa,
Mukherjee Shyamali,
Smith Milton,
Das Salil K
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.710.12
Subject(s) - liposome , antioxidant , mapk/erk pathway , chemistry , pharmacology , vitamin c , kinase , medicine , biochemistry
We reported earlier that antioxidant liposomes containing N‐acetyl cysteine (NAC) and vitamin E protect against CEES‐induced lung injury. Our previous studies also indicated a role of MAPK/AP‐1 signaling in lung injury. The present study examines whether the protection of lung injury by antioxidant liposomes is mediated by a control of MAPKs/AP‐1 signaling. A single dose of the antioxidant liposome (200 μl) was administered intratracheally 5 min after CEES exposure. The animals were sacrificed either 1 h or 30 days after CEES exposure. The treatment caused a down regulation of CEES‐induced activation of TNF‐α. Although, no change was observed in the activation of any member of the MAPKs family, the activation of AP‐1 transcription factors was down‐regulated by the treatment. In addition, the antioxidant liposome treatment caused a decrease in the protein levels of two cell cycle proteins, cyclin D1 and PCNA. This suggests that NAC in combination with vitamin E as a liposome can be used as an effective antidote against lung injury. (Supported by US Army Grant W81XWH‐06‐2‐0044)