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Transcriptional Regulation of the Yeast PAH1 Gene
Author(s) -
Pascual Florencia,
Carman George M.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.689.2
Subject(s) - diacylglycerol kinase , phosphatidate , derepression , dephosphorylation , reporter gene , biochemistry , enzyme , biology , chemistry , gene , phosphatase , gene expression , psychological repression , protein kinase c
The PAH1 gene in Saccharomyces cerevisiae encodes the Mg 2+ ‐dependent phosphatidate (PA) phosphatase. PA phosphatase catalyzes the dephosphorylation of PA yielding diacylglycerol and P i . This enzyme generates the diacylglycerol used for the synthesis of triacylglycerol, and may generate the diacylglycerol used for the synthesis of phospholipids via the Kennedy pathway. The PAH1 ‐encoded enzyme also controls the cellular concentration of its substrate PA, which is the precursor for phospholipids that are synthesized via the CDP‐diacylglycerol pathway. Previous work utilizing a P PAH1 ‐ lacZ reporter gene plasmid expressed in wild type cells showed that PAH1 expression is 5‐fold higher in stationary phase cells than that found in exponential phase cells, and inositol supplementation resulted in a further 2‐fold increase in β‐galactosidase activity in stationary phase cells. Preliminary studies indicated that regulation of PAH1 expression is mediated by the Gis1/Rph1 transcription factors. In this work, the cis ‐ and trans ‐acting elements involved in this regulation are examined. Supported by NIH grant GM 28140.