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Alterations in proteasome plasticity and biogenesis with aging and dietary restriction
Author(s) -
Dasuri Kalavathi,
Keller Jeffrey N.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.668.2
Subject(s) - proteasome , biogenesis , oxidative stress , biology , microbiology and biotechnology , function (biology) , endocrinology , biochemistry , gene
The proteasome is an essential enzyme that appears to be inhibited with aging, although the basis for age‐related impairments in proteasome function remain poorly defined. In the present study we report that aging increases oxidative inactivation, and decreases the stability of the 20S proteasome, with dietary restriction (DR) ameliorating both of these aspects of aging in the liver. Additionally, we have found increased levels of 20S proteasome complexes and evidence for increased levels of proteasome biogenesis in the aging liver, suggesting that during aging tissues may attempt to overcome age‐related increases in oxidative stress via elevating the levels of 20S complexes. Lastly, our studies demonstrate that increased levels of Hsp90 within the 20S proteasome fraction are observed with aging of the liver, which may be an additionally mechanism by which the aging liver attempts to preserve proteasome function during aging. Taken together, these data suggest that the proteasome complex exhibits plasticity in response to aging and DR with regards to proteasome levels, proteasome stability, proteasome biogenesis, and proteasome associated heat shock proteins. These factors may play an important role in regulating the function of the proteasome during aging and in response to beneficial stimuli such as DR.