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EXPRESSION OF PROINFLAMMATORY CHEMOKINES AS BIOCOMPATIBILITY MARKERS OF DENTAL SEALERS
Author(s) -
SOLISSOTO JUAN MANUEL,
SOUSAMORENO VANESSA
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.651.5
Subject(s) - biocompatibility , proinflammatory cytokine , chemokine , ccl3 , dentistry , immunostaining , chemistry , medicine , pathology , inflammation , immunohistochemistry , immunology , ccl2 , organic chemistry
Biocompatibility of new dental sealers should be evaluated before their clinical application. Objective We studied the in situ production of chemokines in order to evaluate the biocompatibility of five endodontic sealers of different composition (Sealer 26, Tubli‐Seal, MTA, Silverfree AH 26, and Endorez). Methods BALB/c mice were subcutaneously implanted with the different dental sealers. Then the tissue was prepared for histopathological and immunohistochemical analysis. Results The proinflammatory chemokines CCL3 and CCL4 participated in both, acute and chronic inflammatory response induced by each dental sealer studied. Immunoreactive cells to both chemokines, were observed abundantly in the dermis, especially in the inflamed tissue around the dental sealers. Higher amounts were observed in the acute inflammatory response. According to morphometrical analysis, the order from more to less biocompatible was: MTA, Sealer 26, Tubli‐Seal, Endorez, Silverfree AH26. There were more immunoreactive cells to CCL4 than CCL3 in response to dental sealers (p<0.05). Conclusions Expression of the proinflammatory chemokines CCL3 and CCL4 is an excellent in vivo model to study the biocompatibility of dental materials. MTA and Sealer 26 are the most biocompatible dental sealers.