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SURFACE MODIFICATION OF METALLIC IMPLANTS TO ENHANCE OSTEOGENESIS
Author(s) -
Patel Shraddha,
Mills David Keith,
Howard Jason,
Lvov Yuri
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.647.7
Subject(s) - osteopontin , osteonectin , osseointegration , osteoblast , materials science , runx2 , chemistry , surface modification , bone morphogenetic protein 2 , surface roughness , titanium , mineralization (soil science) , alkaline phosphatase , osteocalcin , implant , in vitro , biochemistry , composite material , metallurgy , organic chemistry , medicine , surgery , nitrogen , enzyme
Surface topology and chemistry play a role in effecting osseointegration of dental and orthopedic implants with surrounding bone. Titanium dioxide (TiO 2 ) nanofilms, functionalized with BMP‐2, was investigated as an approach to fabricate an instructional surface coating. Six, nine and twelve‐layered TiO 2 substrates were also studied to investigate the additive effect of surface roughness. Osteoblast growth, functionality and matrix mineralization on nanofilms were evaluated. Cell proliferation decreased with increasing surface roughness on both uncoated and BMP‐2 coated TiO 2 substrates as indicated by MTT results. A significant reduction in cell proliferation was also observed on BMP‐2 coated substrates in comparison with uncoated substrates. Osteonectin and osteopontin expression on BMP‐2 coated substrates was significantly greater than that on uncoated substrates suggesting an enhanced osteogenic effect by BMP‐2 on osteoblast protein expression. Varying layers of TiO 2 did not measurably affect osteonectin and osteopontin expression by osteoblasts seeded on both uncoated and BMP‐2 coated substrates. A significant enhancement in calcium production was observed with increasing layers of TiO 2 on both sets of experimental substrates. Increase in calcium levels and deposition on BMP‐2 immobilized substrates showed an enhanced osteogenic effect resulting in increased osteoblast mineralization. This study demonstrated that BMP‐2 could be immobilized onto TiO 2 substrates via nanoassembly and that osteoblast osteogenic activity can be directly influenced by a bioactive substrate. This study suggests that functionalized TiO 2 nanofilms may lead to the development of implants with better osseointegration and enhanced durability.