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Periodontium derived osteoprogenitors isolated from αSMA‐GFP mice expressed bone and stem cell markers
Author(s) -
SANMIGUEL SYMONE MARQUEZ,
Li Haitao,
Igwe John,
Ferrer Vesna,
Aguila Hector,
Kalajzic Ivo
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.647.5
Subject(s) - dental follicle , periodontal fiber , mesenchymal stem cell , alkaline phosphatase , bone sialoprotein , biology , microbiology and biotechnology , chemistry , osteocalcin , dentistry , medicine , biochemistry , enzyme
Purpose This study was conducted to determine if the αSMA promoter would direct GFP expression in osteoprogenitor cells of dental follicle and periodontal ligament. Methods Epifluorescence was evaluated in histological sections of mandibles derived from neonatal and 4‐6 week old mice. Transgene expression was also evaluated in primary cultures derived from dental follicle (mDF) and periodontal ligament (mPDL). Osteogenic differentiation was determined by RNA expression of bone markers, histochemical staining for alkaline phosphatase (ALP) and detection of mineralized nodules by xylenol orange. Flow cytometry (FACS) was used to determine cellular profile of freshly isolated or cultured αSMA positive cells. Results αSMA‐GFP expression was observed in the dental follicle, and in apical regions of the root. Strong expression of αSMA‐GFP was observed during early culture stages and diminished as mineralization progressed. Intense ALP activity, presence of mineralized nodules and increased expression of bone sialoprotein and dentin matrix protein‐1 following two weeks of osteogenic induction was observed. FACS analysis revealed that freshly digested mDF cells expressed the mesenchymal markersThy‐1 (40%) and Sca‐1 (8.79%). Conclusion αSMA promoter has the potential to identify a population of mesenchymal progenitor cells residing within the dental follicle and periodontal ligament. Grant Funding Source NIH GRANTU24DE06495