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Dynamic Analysis of Blood Vessel Formation in Transgenic Quail Embryos
Author(s) -
Sato Yuki,
Huss David,
Poynter Greg,
Fraser Scott E,
Lansford Rusty
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.643.2
Subject(s) - biology , microbiology and biotechnology , quail , dorsal aorta , angiogenesis , blood vessel , live cell imaging , anatomy , stem cell , cell , genetics , endocrinology , haematopoiesis
Blood vessels help to establish the circulatory system essential for gas, nutrient, and waste exchange. Blood vessels also play a vital role in formation of the nervous system and visceral organs during development. Although numerous molecules required for blood vessel formation have been identified, a detailed understanding of normal blood vessel formation and direct effects of the gene defects is lacking due to the difficulty of imaging living embryos. To take advantage of the easy accessibility to the developing embryos, we generated Tie1.H2B‐EYFP transgenic quail that express H2B‐eYFP in endothelial cells. The Tie1.H2B‐EYFP transgenic quail enable us to image nucleus of every blood vessel endothelial cell in living embryo by EYFP signals under the confocal laser microscopes. We are studying aorta formation to better understand various types of dynamic morphogenetic events including cell migration/sprouting, cell division, mesenchymal‐to‐epithelial transition, and rearrangement of luminal structure. We dynamically track individual endothelial cells to determine their individual behavior and their collective morphometric changes during the dorsal aorta formation. By combining dynamic imaging with DNA electroporation technique, we are trying to visualize the functional role of Shh signaling for the endothelial cell in angiogenesis and cardiogenesis. Grant Funding Source NIH, Center of Excellence in Genomic Science (CEGS), Japan Society for Promotion of Science (JSPS)