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Effect of Fluid Shear Stress on Embryonic Stem Cells
Author(s) -
Ahsan Taby,
Doyle Adele M,
Nerem Robert
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.638.3
Subject(s) - embryonic stem cell , stem cell , matrigel , microbiology and biotechnology , shear stress , endothelial stem cell , biology , in vitro , andrology , chemistry , medicine , materials science , gene , genetics , composite material
Physical forces, such as compression, tension, and shear, have long been applied to differentiated cells. The effects of applied mechanical cues on stem cell self‐renewal and differentiation, however, have yet to be well characterized. In these studies, we focus on the effects of applied fluid shear stress on mouse embryonic stem cells. Previously, we had shown that the application of 15 dynes/cm/cm of shear stress to mouse embryonic stem cells significantly (p<0.05) increased protein expression for endothelial markers (e.g. Flk1, VECAD, PECAM) and promoted endothelial functionality (structure formation on Matrigel(tm) in vitro). Recent mRNA expression studies have found that the application of shear over a range of magnitudes (5‐15 dynes/cm/cm) resulted in similar changes on embryonic stem cell differentiation in Flk1, FLT, VECAD, and PECAM. towards an endothelial phenotype. Changes in magnitude, however, differentially regulate (p<0.05) genes associated with atherosclerosis, such as PPARG and VCAM1. The authors would like to acknowledge the Parker H. Petit Institute for Bioengineering & Bioscience and the NSF ERC "Georgia Tech/Emory Center for Living Tissues" for funding.