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The diversity and distribution of lymphatic endothelial cell markers as identified by monoclonal antibodies
Author(s) -
Ezaki Taichi,
Shimizu Kazuhiko,
Morikawa Shunichi,
Kitahara Shuji
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.636.1
Subject(s) - monoclonal antibody , antigen , lymphatic system , immunohistochemistry , biology , antibody , pathology , lymphatic endothelium , immunology , medicine
Recently, various novel lymphatic endothelial cell (LEC) markers have been discovered and our knowledge about lymphatic system has been accumulated. However, the distribution of each marker antigen does not necessarily coincide with each other. This may even cause a great confusion for the differentiation of LECs from other cell lineages, such as blood endothelial cells (BECs), lymphoid cells, and so on. In this study we focused on the diversity of LEC markers by the immunohistochemical characterization of the antigen distribution with monoclonal antibodies (mAbs). We have produced a novel mAb, LA102, reacting predominantly with mouse LECs (Ezaki et al., 2006) by the immunization with adjuvant‐induced benign mouse lymphangioma cells. It recognizes an about 26 kD protein antigen. We compared its immunoreactivity with those of other antibodies to LEC markers and found that the distribution of the markers varied depending on their tissue localization or various pathological conditions. This work was mainly supported by a Grant‐in‐Aid for Scientific Research (B) (No.19390053) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.