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MicroRNA Expression Profile in Bronchopulmonary Dysplasia
Author(s) -
Bhaskaran Manoj,
Wang Yang,
Breshears Melanie A,
Liu Lin
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.630.4
Subject(s) - bronchopulmonary dysplasia , microrna , pathogenesis , hyperoxia , microarray , lung , medicine , disease , bioinformatics , microarray analysis techniques , biology , pathology , gene expression , gene , genetics , pregnancy , gestational age
MicroRNAs (miRNAs) are small RNAs with a length of ~21‐24 nucleotides. They are endogenous in origin and regulate the expression of proteins post‐transcriptionally. miRNA expression profile in a disease provides valuable insights into the pathogenesis of that disease and possible therapeutic strategies. Bronchopulmonary dysplasia (BPD) is the chronic lung disease manifested in premature infants and has multifactorial etiology ranging from hyperoxia exposure to premature lung to neonatal therapeutics. Advancement in treating premature infants using new management and therapeutic strategies has changed the course of BPD pathogenesis. In this study we used a 'new' BPD model where the primary lesions have shifted from chronic inflammatory responses, as seen in 'old' BPD models, to actual dysplasia. Infant rats were exposed to 95% oxygen from postnatal day 3 to day 14. The time point selected showed changes in the lung that mimicked the new BPD pathology. Small RNA fraction was isolated and miRNA microarray was performed on BPD and control samples. We found that five miRNAs, miR‐342, miR‐335, miR‐150, miR‐126* and miR‐151* were down‐regulated and three miRNAs, miR‐21, miR‐141 and miR‐34a were up‐regulated in the BPD lung. Most of these miRNAs have target proteins vital to normal lung development and the study thus provide us with new insights into miRNA‐mediated regulation of BPD pathogenesis.