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Potential Effect of β‐Estradiol in Human Jurkat T‐Cells
Author(s) -
Hullitt JoAnna,
Davis Corey,
Yedjou Clement,
Cameron Joseph A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.626.7
Subject(s) - jurkat cells , trypan blue , estrone , estrogen , cell growth , mtt assay , estriol , viability assay , chemistry , cell , endocrinology , medicine , biology , microbiology and biotechnology , t cell , immunology , biochemistry , immune system
β‐estradiol is the most potent estrogen of a group of endogenous estrogen steroids which includes estrone and estriol. This steroid hormone is the most potent natural estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Although β‐estradiol protects the renal and cardiovascular systems, the mechanisms involved remain unclear. In this research, we performed both MTT assay and trypan blue exclusion test to: 1. evaluate the effect of β‐estradiol in HL‐60 and Jurkat T‐cells and 2. compare the sensitivity of these two cells types. The results from both MTT assay and trypan blue exclusion test demonstrated that low, physiological levels of β‐estradiol induce cellular proliferation in Jurkat T‐cells. At higher dose of exposure (16 uM), β‐estradiol decreases the viability of Jurkat T‐cells compared to the control cells. A similar trend was obtained with the trypan blue exclusion test using the hemacytometer to count the cells manually. In summary, the results of the present study demonstrate that physiological levels of β‐estradiol induce cell growth, and cellular proliferation of Jurkat T‐cells, whereas higher doses inhibit cell growth and induce cell death. Supported in part by NIGMS‐NIH Grant R25 GM50117 and RCMI‐NIH Grant G12RR13459.