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Involvement of NF‐kappaB in the Cross Talk Between the Renin‐Angiotensin System and Insulin Signaling in Skeletal Muscle in Burn Injury
Author(s) -
Wayne Michelle Marie,
Lepard Jacob,
Benavides Jeshurun,
West Lori Gail,
Enderson Blaine,
Daley Brian,
Kasper Sherry Oden,
Karlstad Michael
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.626.19
Subject(s) - losartan , endocrinology , burn injury , medicine , insulin receptor , insulin , insulin resistance , renin–angiotensin system , skeletal muscle , angiotensin ii , total body surface area , receptor , surgery , blood pressure
Upregulation of the renin angiotensin system (RAS) is involved in impaired insulin signaling and glucose intolerance in type 2 diabetes and burn injury. We showed that losartan, an AT 1 receptor blocker, improved insulin signaling and glucose tolerance in burn‐injured rats. It has been shown that NF‐kappaB activation may play an important role in angiotensin II induced skeletal muscle insulin resistance. Our aim was to examine the possible mechanism of the cross talk between the RAS and insulin signaling systems in skeletal muscle in burn injury. To assess the involvement of NF‐kappaB, we measured the levels of IkappaB, an inhibitor of NF‐kappaB, in rectus abdominus muscle three days after sham or burn injury. A 30% body surface area burn was induced in male Sprague‐Dawley rats by immersion of the dorsum into 90C water for 15s. Losartan (30 mg/kg/day) or placebo (water) was given by gavage for three days post‐burn. The level of IkappaB decreased in burned rats given placebo but returned to sham placebo levels in burned rats given losartan. These data suggest that NF‐kappaB may be involved in the cross talk between the renin‐angiotensin system and insulin signaling in skeletal muscle in burn injured rats.