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Differences between two inbred rat strains in number of neurons expressing K+ ion channels in the medullary raphe nucleus (MRN)
Author(s) -
Riley Desire,
Neumueller Suzanne,
Bonis Josh,
Dwinell Melinda,
Qian Baogang,
Forster Hubert
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.621.4
Subject(s) - nissl body , chemistry , medicine , endocrinology , raphe nuclei , biology , microbiology and biotechnology , anatomy , serotonin , staining , genetics , receptor , serotonergic
Male (M) and female (F) Dahl salt sensitive (SS) and Brown Norway (BN) inbred rat strains increase pulmonary ventilation by 183 and 37% respectively when exposed to 7% inspired CO 2 . Since K + ion channels are hypothesized determinants of ventilatory CO 2 sensitivity, we presently determined whether there is a difference between these two rat strains in number of neurons expressing K + channels in the chemosensitive MRN. Rats were sacrificed, and the brains were perfused, extracted, cyrosectioned at 25μm into 4 series, and alternate sections were stained for Nissl and Task‐1, K v 1.4, or K ir 2.3 K + channel antibodies. From 0‐2mm rostral to obex, the stained neurons in the MRN were microscopically visualized and counted. We found more Nissl stained MRN neurons in BN than in SS rats in both M (6%, P<0.001) and F (8.2%, P<0.001) rats. However, for Task‐1, K v 1.4, and K ir 2.3, there were 12, 13.8, and 13.9% respectively more (P<0.001) immunostained MRN neurons in SS than in BN rats. Additionally, F BN rats had 5.3 (P=0.014), 7.3 (P=0.040), and 7.6% (P=0.005) more Task‐1, K v 1.4, and K ir 2.3 respectively stained MRN neurons than in F SS rats. We conclude that the number of neurons expressing K + channels in the MRN is genetically determined. Supported by NIH 25739, HL066579, and the Department of Veterans Affairs.