Premium
Effects of allopurinol on plasma uric acid concentrations and leukocyte oxidative activity in broilers
Author(s) -
Carro Maria Dolores,
Falkenstein Elizabeth,
VanDyke Knox,
Klandorf Hillar
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.617.7
Subject(s) - uric acid , allopurinol , xanthine oxidase , oxidative stress , chemistry , xanthine , oxidative phosphorylation , antioxidant , xanthine oxidase inhibitor , medicine , endocrinology , glutathione , zoology , body weight , biochemistry , biology , enzyme
Allopurinol (AL) is a xanthine oxidase inhibitor that has been recently proposed as a therapy to reduce oxidative stress in human tissues. The effects of xanthine oxidase on oxidative stress are complex, since it forms uric acid (a potent antioxidant) but also catalyzes the formation of reactive oxygen species (ROS). This study was designed to investigate the effects of AL on plasma uric acid (PUA) concentrations and oxidative activity in broilers. Fifteen 5 wk‐old male chickens were divided into 3 groups, and each was randomly assigned to one of the following treatments: CON (control), AL25 (25 mg AL/kg body weight (BW)) and AL50 (50 mg AL/kg BM). Birds were weighed twice weekly for 4 wks, feed intake was measured every two days, and leukocyte oxidative activity (LOA) and PUA levels were determined weekly. Feeding AL decreased feed intake in both groups (P < 0.01), and by wk 4 reduced (P < 0.005) BW to 86.9 and 73.2% of that in CON group for AL25 and AL50 birds, respectively. As expected, AL reduced (P < 0.001) PUA at each wk, mean values being 41.8 and 36.3% of those in CON for AL25 and AL50, respectively. There was no effect (P > 0.05) of AL on LOA over the first 3 wk, but at wk 4 LOA in AL25 birds was 4.6 times greater (P = 0.008) than that in CON. The general lack of effect of AL on LOA would indicate a decrease in xanthine oxidative activity associated with a reduction in ROS production and growth rate. Support M.D. Carro had a Spanish MICIN scholarship