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The effects of an antioxidant cocktail on apoptotic signaling in the soleus muscles of aged hindlimb suspended rats
Author(s) -
Nichols Cody E,
Jackson Janna R,
Regal Steve M,
Alway Stephen E
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.617.6
Subject(s) - hindlimb , atrophy , apoptosis , soleus muscle , muscle atrophy , oxidative stress , blot , chemistry , caspase 3 , medicine , pharmacology , endocrinology , pathology , biochemistry , skeletal muscle , programmed cell death , gene
Hindlimb suspension is a technique used in rats to simulate non‐weight bearing situations. In using this technique the hindlimbs atrophy. It is known that slow twitch muscles atrophy severely under hindlimb suspension. It has also been shown that oxidative stress could be a possible cause of apoptosis, and apoptosis is a cause of atrophy. This experiment was conducted to see if by using an antioxidant cocktail the amount of apoptosis and atrophy could be decreased. This was accomplished by orally gavaging the rats with compound: seven days before suspension and while being suspended for fourteen days. The rats were then sacrificed and the right soleus was removed in vivo. Many apoptotic markers were then found using a western blotting technique. After running an ANOVA on the data obtained by a caspase assay, it was shown that the suspension had an effect on caspase 3 activity and supplementation had an effect on caspase 9 and caspase 3 activity. (Supported by NIH Grant 5P20RR016477 to the West Virginia IDeA Network for Biomedical Research Excellence)