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Protective effect of hypothermia and mannitol postconditioning on mouse brain suffered from ischemic/reperfusion injury
Author(s) -
SHEN Jia,
LI Menghan,
BAI Xiaoyin,
XIA Qiang
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.614.17
Subject(s) - mannitol , hypothermia , medicine , anesthesia , ischemia , reperfusion injury , hippocampus , nissl body , neuron , pharmacology , chemistry , staining , biochemistry , pathology , psychiatry
OBJECTIVE The aim of the study was to determine the synergistic protective effect of mild hypothermia combined mannitol therapy against global cerebral ischemia/reperfusion (I/R) injury and the underlying mechanisms. METHODS Male C57BL/6 mice (22‐28g) were randomly divided into sham group (Gourp S), control (ischemia/reperfusion) group (Group C), mild hypothermia (31‐33°C, 2h after reperfusion) group (Group Ht), mannitol (20% mannitol, caudal intravenous infusion) group (Group Ma), mild hypothermy combined mannitol group (Group Ht+Ma). Learning and memory abilities were detected by Morris water maze task, the CA1 region neuron survival rates were detected by Nissl staining, the activities of superoxide dismutase (SOD) of hippocampus tissue were measured. RESULTS The average escape latency and swimming distance of Group Ht+Ma were significantly shorter than Group C, also shorter than those of Group Ht and Group Ma. The average neuron survival rates were markedly increased in Group Ht+Ma, Group Ht and Group Ma, more than that in Group C. The SOD activity of Group Ht+Ma was remarkably higher than control group, and higher than group Ht and group Ma. CONCLUSION The therapies of mild hypothermia and mannitol have protective effect against global ischemia/reperfusion injury. The combination of mild hypothermia and mannitol therapy enhances the protection effect by increasing the SOD activity.